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All tumor samples utilised had been established to get >80% tumor by analysis of H&E-stained sections. H&E-stained sections of each usual sample were being evaluated and identified to deficiency any precancerous lesions. Clinic pathologic functions are outlined in Supplementary desk two. Samples for immunoblotting were frozen at −80 °C and those utilized for qRT-PCR ended up right away immersed in RNA later on stabilization Answer (Ambion–everyday living Technologies, Carlsbad, CA, USA) and saved at 4 °C overnight, then samples had been moved to −eighty °C for long-time period storage. This examine was performed with acceptance in the Brazilian National Cancer Institute’s Ethic Committee (Registration amount: 84/04).
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